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Phosphorylation is among the most widespread post-translational modifications in nature, and it has been estimated that more than 30% of the proteins in a given mammalian cell at some point during their expression are phosphorylated. Phosphorylation and dephosphorylation of proteins regulates a large number of biological processes such as signal transduction, molecular recognition and interaction, and other cellular events. Phosphoproteome mapping can be challenging due to low abundance, dynamic and much less ionization efficiency.

The Preciex® Phosphopeptide enrichment Stage-tip uses a TiO2 Resin with optimized protocol and buffers resulting in a higher yield of phosphopeptides ready for direct MS analysis without the need for additional clean up. It enhances the ability to routinely identify and characterize large numbers of phosphorylated species within complex protein mixtures. Process of use: centrifugation, vacuum, positive pressure.Formats: 10µL and 200µL.

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  *200µL tips are available for different Liquid Handling Automation systems (Zymark, Multiprobe, Tecan, Hamilton, Gilson, Qiagen, and many more) without any further adaptor needed.  

Features of Phosphopeptide Stage-tip:

• Higher selectivity for multiply Phosphorylate peptides
• Achieve ready-to-measure Phosphopeptides

• Suitable for manual and automated processing

Common product Specifications:

Sample	Phosphopeptide
Chromatographic bed	TiO2 resin
Volume	200μL
Capacity	4ug
PH Range	1-14
Temperature	4-40°C
Quantity	96 Tips
Detection Method	HPLC, Mass spectrometr꧙y, Capillary electrophoresis, Atomic abso🐈rption spectroscopy

Ordering Information:

Description	Format	Capacity	Part Number
Preciex Phosphopeptide	10μL	1μg	2108011
Preciex Phosphopeptide	200μL	4μg	2108042
Preciex Phosphopeptide	200μL	15μg	2108153

 

User Guide

Material Safety Data Sheets (MSDS)